Dr. Magnús Örn Stefánsson

Major technical skills

Molecular Biology, Genetics & Biochemistry

  •  DNA extractions (various methods, chemical and sonication)
  •   DNA amplification using PCR
  •   Agarose gel electrophoresis
  •   Polyacrylamide gel electrophoresis
  •   Extensive experience in the use of an automated DNA analyser and associated DNA analysis software
  •   Microsatellites screening and allele scoring
  •   Pan I locus screening
  •  Restriction enzymes
  •   Magnetic particles, hybridization of target DNA
  •   DNA sequencing (LiCor and AIB)
  •   Genomic library construction, molecular cloning, and Southern blotting
  •   Development of novel microsatellite loci
  •  Amperometric detection of DNA molecules through reduction-oxidation reaction (redox)
  •   Development of DNA affinity biosensors
  •   Enzyme kinetics
  •  Gas chromatography (GC)
  • Antioxidant measurements
  • Protein measurements
  • Cell culture
  • Chlorophyll measurements

Haematology & Physiology

Methods:

  • Blood sampling
  • Haematocrit
  • Leucocrit
  • Haemoglobin
  • Plasma electrolytes
  • Osmolarity
  • Hormones
  • GLP & SOP 

Instrumentation:

  • Culture counter
  • Spectrophotometer
  • ELISA
  • Pipette robot
  • Flame photometer
  • Osmometer
  • Microbial biosensors
  • Chloride titrator
  • Radioimmuno-assay

Husbandry & Rearing

  • Fish Culture
    • Larvae
    • Juveniles
    • Broodstock
  • Fish tagging
  • Morphometrics
  • Phyto-and zoo-plankton culture: 
    • Microalgae
    • Rotifers
    • Artemia
  • Monitoring and running of recirculation units for fish culture
  • Biofiltration / water quality measurements:
    • Ammonia
    • Nitrate
    • Nitrite
    • Oxygen
    • pH
    • BOD
    • Salinity